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The comfortable-clipped sequences that overhang the breakpoint are collapsed right into a consensus sequence employing a a number of sequence alignment (MSA) supplied in the seqAn library [27]. Wham applies a few filters towards the consensus sequences. Breakpoints will not be claimed in scenarios in which consensus sequences are shorter than ten b
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Wham is made for paired-conclusion Illumina libraries with regular insert measurements (~300bp-500bp). Wham integrates mate-pair mapping, break up browse mapping, smooth-clipping, alternate alignment and consensus sequence centered evidence to predict SV breakpoints with one-nucleotide precision. Wham generates a mixed pileup (catalog of reads mask